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origene qstar  (OriGene)


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    Structured Review

    OriGene origene qstar
    Origene Qstar, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+origene/Gapdh+Mouse+qPCR+Primer+Pair/pm41881279-119-11-11
    Average 94 stars, based on 29 article reviews
    origene qstar - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: A new role for human dyskerin in vesicular trafficking
    Article Snippet: anti‐dyskerin Santa Cruz Biotechnology, Dallas, TX, USA sc‐48794 WB/IF anti‐PARP 1 Santa Cruz Biotechnology, Dallas, TX, USA sc‐7150 WB anti‐GAPDH Origene, Rockville, MD, USA TA308884 WB anti‐p21 Origene, Rockville, MD, USA TA307018 WB anti‐caspase 3 Millipore, Billerica, MA, USA 04‐439 WB anti‐Rab 11 A Abcam, Cambridge, UK ab65200 WB/IF anti‐Rab 5 Santa Cruz Biotechnology, Dallas, TX, USA ab18211 WB/IF anti‐CD63 Abcam, Cambridge, UK ab8219 IF anti‐β‐tubulin Hybridoma bank, Iowa City, USA E7‐s IF Cy3‐conjugated anti‐goat IGG Bethyl, Montgomery, USA A50‐201C3 IF FITC‐conjugated anti‐rabbit IGG Bethyl, Montgomery, USA A120‐100F IF .. anti‐dyskerin Santa Cruz Biotechnology, Dallas, TX, USA sc‐48794 WB/IF anti‐PARP 1 Santa Cruz Biotechnology, Dallas, TX, USA sc‐7150 WB anti‐GAPDH Origene, Rockville, MD, USA TA308884 WB anti‐p21 Origene, Rockville, MD, USA TA307018 WB anti‐caspase 3 Millipore, Billerica, MA, USA 04‐439 WB anti‐Rab 11 A Abcam, Cambridge, UK ab65200 WB/IF anti‐Rab 5 Santa Cruz Biotechnology, Dallas, TX, USA ab18211 WB/IF anti‐CD63 Abcam, Cambridge, UK ab8219 IF anti‐β‐tubulin Hybridoma bank, Iowa City, USA E7‐s IF Cy3‐conjugated anti‐goat IGG Bethyl, Montgomery, USA A50‐201C3 IF FITC‐conjugated anti‐rabbit IGG Bethyl, Montgomery, USA A120‐100F IF anti‐rabbit Bethyl, Montgomery, USA A120‐100P WB DyLight 550 Phalloidin Thermo Fisher Scientific, Waltham, MA, USA 21835 IF ..

    Article Title: A new role for human dyskerin in vesicular trafficking
    Article Snippet: .. anti‐dyskerin Santa Cruz Biotechnology, Dallas, TX, USA sc‐48794 WB/IF anti‐PARP 1 Santa Cruz Biotechnology, Dallas, TX, USA sc‐7150 WB anti‐GAPDH Origene, Rockville, MD, USA TA308884 WB anti‐p21 Origene, Rockville, MD, USA TA307018 WB anti‐caspase 3 Millipore, Billerica, MA, USA 04‐439 WB anti‐Rab 11 A Abcam, Cambridge, UK ab65200 WB/IF anti‐Rab 5 Santa Cruz Biotechnology, Dallas, TX, USA ab18211 WB/IF anti‐CD63 Abcam, Cambridge, UK ab8219 IF anti‐β‐tubulin Hybridoma bank, Iowa City, USA E7‐s IF Cy3‐conjugated anti‐goat IGG Bethyl, Montgomery, USA A50‐201C3 IF FITC‐conjugated anti‐rabbit IGG Bethyl, Montgomery, USA A120‐100F IF .. anti‐dyskerin Santa Cruz Biotechnology, Dallas, TX, USA sc‐48794 WB/IF anti‐PARP 1 Santa Cruz Biotechnology, Dallas, TX, USA sc‐7150 WB anti‐GAPDH Origene, Rockville, MD, USA TA308884 WB anti‐p21 Origene, Rockville, MD, USA TA307018 WB anti‐caspase 3 Millipore, Billerica, MA, USA 04‐439 WB anti‐Rab 11 A Abcam, Cambridge, UK ab65200 WB/IF anti‐Rab 5 Santa Cruz Biotechnology, Dallas, TX, USA ab18211 WB/IF anti‐CD63 Abcam, Cambridge, UK ab8219 IF anti‐β‐tubulin Hybridoma bank, Iowa City, USA E7‐s IF Cy3‐conjugated anti‐goat IGG Bethyl, Montgomery, USA A50‐201C3 IF FITC‐conjugated anti‐rabbit IGG Bethyl, Montgomery, USA A120‐100F IF anti‐rabbit Bethyl, Montgomery, USA A120‐100P WB DyLight 550 Phalloidin Thermo Fisher Scientific, Waltham, MA, USA 21835 IF



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    Study design for RNA extraction and PCR methods. The figure outlines the process used in the study of RNA extraction and PCR methods from stool samples in two cohorts: an evaluation cohort (24 samples: 9 cancer, 8 polyps, 7 controls) and a test cohort (68 samples: 22 cancer, 24 polyps, 22 controls). The evaluation cohort involved multiple RNA extraction methods: Norgen (N), Qiagen (Q), and EasyMAG BioMérieux (EM), with subsequent quality control steps using cel-mir-39 and Oligo-IC mRNA. The PCR methods included two-step RT and GAPDH PCR with a DNA intercalating dye (SYBR green) and TaqMan probes, and one-step GAPDH RT-PCR with TaqMan probes. In the test cohort, RNA extraction was performed using Norgen (N), followed by one-step RT-PCR with TaqMan probes targeting GAPDH, CXCL1, IL18, IL1B, IL6, PTGS2 , and SPP1 .

    Journal: Scientific Reports

    Article Title: Selection of optimal extraction and RT-PCR protocols for stool RNA detection of colorectal cancer associated immune genes

    doi: 10.1038/s41598-024-78680-0

    Figure Lengend Snippet: Study design for RNA extraction and PCR methods. The figure outlines the process used in the study of RNA extraction and PCR methods from stool samples in two cohorts: an evaluation cohort (24 samples: 9 cancer, 8 polyps, 7 controls) and a test cohort (68 samples: 22 cancer, 24 polyps, 22 controls). The evaluation cohort involved multiple RNA extraction methods: Norgen (N), Qiagen (Q), and EasyMAG BioMérieux (EM), with subsequent quality control steps using cel-mir-39 and Oligo-IC mRNA. The PCR methods included two-step RT and GAPDH PCR with a DNA intercalating dye (SYBR green) and TaqMan probes, and one-step GAPDH RT-PCR with TaqMan probes. In the test cohort, RNA extraction was performed using Norgen (N), followed by one-step RT-PCR with TaqMan probes targeting GAPDH, CXCL1, IL18, IL1B, IL6, PTGS2 , and SPP1 .

    Article Snippet: 250 ng RNA*. iScript cDNA synthesis kit (Biorad). 20 μl volume. Random hexamer primers. , 2 μl undiluted cDNA (approx. 25 ng) Quantinova SYBR Green PCR Kit (Qiagen) , Two-step RT-PCR. RT: 25° C 5 min, 46 °C, 20 min, 95 °C 1 min. PCR: 40 cycles of 5 Sect. 95 °C, 10 Sect. 60 °C. , SYBR green GAPDH : Origene Qstar NM002046 F: 5’ GTCTCCTCTGACTTCAACAGCG-3 R: 5’ACCACCCTGTTGCTGTAGCCAA-3’ (spans exons 7–8).

    Techniques: RNA Extraction, Control, SYBR Green Assay, Reverse Transcription Polymerase Chain Reaction, One Step RT-PCR

    Comparison of extraction methods and PCR methods (Dunn’s test).

    Journal: Scientific Reports

    Article Title: Selection of optimal extraction and RT-PCR protocols for stool RNA detection of colorectal cancer associated immune genes

    doi: 10.1038/s41598-024-78680-0

    Figure Lengend Snippet: Comparison of extraction methods and PCR methods (Dunn’s test).

    Article Snippet: 250 ng RNA*. iScript cDNA synthesis kit (Biorad). 20 μl volume. Random hexamer primers. , 2 μl undiluted cDNA (approx. 25 ng) Quantinova SYBR Green PCR Kit (Qiagen) , Two-step RT-PCR. RT: 25° C 5 min, 46 °C, 20 min, 95 °C 1 min. PCR: 40 cycles of 5 Sect. 95 °C, 10 Sect. 60 °C. , SYBR green GAPDH : Origene Qstar NM002046 F: 5’ GTCTCCTCTGACTTCAACAGCG-3 R: 5’ACCACCCTGTTGCTGTAGCCAA-3’ (spans exons 7–8).

    Techniques: Comparison, Extraction, SYBR Green Assay